巴西橡胶树CAMTA转录因子全基因组鉴定与表达分析.docx

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1、巴西橡胶树CAMTA转录因子全基因组鉴定与表达分析林显祖，肖小虎，阳江华，秦云霞，龙翔宇，方永军 1 HbCAMTA基因的理化性质分析Tab. 1 Physicochenucal properties of HbC AMTAs斌因名称Gene name级因IDGene ID内含于教日Intronnumber量内序列长度Proteinlength aa分子量Molecularweigh kD等电点Isoelectncpoint结构域倏测Predictedpfam domain节细胞定位Subcellularprediction总y均嫉水性Grand average ofhydropathicih

2、,信号肽*测SingalpetidesHbCMTAlscaffoid0625-l 1772712923104 346.45CG-1 domainNucleus-0.439NAHbCAS/TAJscaffold070361008S11991111 307.83CG-1 domainNucleus-0.522NAHbCMTASscaffold0010_4040802121069120855.63CG-1 domainNucleus-0 546NAHbC4MTA4scaffold0824J4043512983110 415.32CG-1 domainNucleus-0574NAHbCiTA5scaf

3、fold0444_896392121086121995.92CG-1 domainNucleus-0.53NAHbClTA6scaffold0711J688712974109.515.92CG-1 domainNucleus-0533NAHbCfTA7scaffold06216051612928104.706.42CG-1 domainNucleus-0384.NAHbCMTA8scaffold0909J93686121068119 945 46CG-1 domainNucleus-0 502NA摘 要:钙调蛋白结合转录激活因子(Calmodulin-binding transcription

4、activator, CAMTA)是一类存在于真核生物中结构保守的重要钙调素结合蛋白，广泛参与植物的胁迫应答、生长发育及激素信号转导等生物学过程。本文以巴西橡胶树(Hevea brasiliensis)为研究对象，从全基因组水平鉴定到8个橡胶树HbCAMTA基因，并对这些基因的理化性质、基因结构、表达模式、启动子区调控元件进行分析。结果显示，该基因家族所有成员均定位于细胞核内，编码蛋白均为亲水性蛋白，分子量在104.34121. 99 kD之间，等电点在5. 327. 83之间；家族基因根据蛋白序列同源性可以分成4个亚组，亚组成员在基因结构、保守基序数目和相对位置等方面具有较高相似性。调控Hb

5、CAMTA的miRNA预测分析显示，每个家族成员受到至少3个miRNA调控。表达分析结果显示，HbCAMTA在不同组织的表达有明显的差异，在叶片中随着叶片的发育表达不断上调，在成熟期或变色期达到最高的表达水平；除HbCAMTA2外，乙烯刺激未能显著诱导橡胶树其他CAMTA基因的表达，而低温可以显著诱导HbCAMTA的上调表达。HbCAMTAl是醪乳、叶片、根、树皮、雄花、雌花以及叶片整个发育过程(古铜期、变色期、淡绿期、成熟期)中最高表达的基因家族成员，表明其具有非组织特异性的基础生物学功能。HbCAMTA3在种子中表达水平最高且受低温胁迫诱导显著上调表达，表明其在响应低温胁迫过程中具有重要的

6、作用。IIbCAMTA7在大多数组织、乙烯处理、低温胁迫处理以及叶片不同发育时期均是最低表达。本研究初步揭示了橡胶树CAMTA家族成员的理化特征和表达特性，为进一步阐明该基因功能奠定了重要的基础。Key：巴西橡胶树；转录因子;CAMTA；基因表达：S794. 1： AGenome-wide Identification and Expression Analysis of the CAMTAFamily in Rubber Tree (Hevea brasiliensis)LIN Xianzul, 2, XIAO Xiaohu2, YANG Jianghua2, QIN Yunxia2, LO

7、NGXiangyu2, FANG Yongjun2*1. College of Tropical Crops, Hainan University, Haikou, Hainan570228, China； 2. Key Laboratory of Biology and Genetic Resources ofRubber Tree, Ministry of Agriculture and Rural Affairs / Instituteof Rubber Research, Chinese Academy of Tropical AgriculturalSciences, Haikou,

8、 Hainan 571101, ChinaAbstract： Calmodulin-binding transcription activators (CAMTAs) areimportant proteins with a conserved calmodulin- binding domain,which are widely involved in biological processes such as stressresponse, growth and development, and hormone signalling in plant.In this study, we id

9、entified eight IlbCAMTAs screening the genome-wide of the rubber tree and analyzed physicochemical properties,gene structures, regulatory elements, gene expression patterns,and so on. The results showed that al1 HbCAMTAs were located in thenucleus, and predicted as hydrophilic proteins with molecula

10、r weightfrom 104. 34 to 121. 99 kD and isoelectric points between 5. 32 and7. 83. The sequence homology analysis showed that HbCAMTAs could bedivided into four subclades, and each one had similar genestructure, conserved domains. Furthermore, each IlbCAMTA waspotentially regulated by at least three

11、miRNAs. Expression analysisindicated that the expression of HbCAMTAs differed significantly indifferent tissues, and the expression was continuously up-regulatedin leaves with the development of leaves and reached the highestexpression level at mature or palegreen. Except for HbCAMTA2,ethylene stimu

12、lation failed to significantly induce the expression ofother CAMTA genes in rubber tree whi1e low-temperature couldsignificantly induce the up-regulated expression of HbCAMTAs.HbCAMTAl was constitutively expressed in all samples, and alsomostly expressed in latex, leaves, roots, bark, male and femal

13、eflowers, and four different leaf developmental stages (Bronzite,Changecolor, Palegreen, and Mature) , which suggesting itsfundamental function in rubber tree. HbCAMTA3 was expressed at thehighest level in the seeds and was significantly up-regulated by lowtemperature stress, indicating its importan

14、t role in response to lowtemperature stress. HbCAMTA7 was undetectably express in allinvestigated samples including different tissues, leafdevelopments, and treatments. Finally, this study would provideimportant information for further revealing the function of HbCAMTAsin rubber tree.Keywords： Hevea

15、 brasiliensis； transcription factor； CAMTA； geneexpressionDOI： 10. 3969/j. issn. 1000-2561. 2021. 10. 015钙离子(Ca2+)是一种广泛存在于植物中的重要的第二信使，在植物的生长发育和胁迫响应的信号转导中发挥着重要的作用。钙调蛋白(Calmodulin, CaM)作为植物中重要的钙离子(Ca2+)结合蛋白之一，广泛存在于植物当中。CaM在细胞中主要负责感知Ca2+浓度变化，进而达到传递Ca2+信号的目的，从而在诸多生理生化调节过程中扮演重要的角色1。Ca2+-CaM信号系统在植物调控抗病抗逆过程中起着重要作用，参与活性氧的产生2、过敏性坏死反应3、蛋白质磷酸化过程及PR基因表达4的调节。目前，植物中已经鉴定出90多个转录因子为Ca2+/CaM结合蛋白，其中包括CAMTA、MYB、WRKY