测量应用案例63200SM.docx

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1、测量应用案例-63-200SM文献名：Inf1uenceof2-(diisopropy1amino)ethy1methacry1ateonacid-triggeredhydro1ysisofcyc1icbenzy1ideneaceta1sandtheirimportanceinefficientdrugde1ivery作者：MinjieTang,aZhengYang,aZujianFeng,aJunhuiZhou,aJinjian1iu?Jianfeng1iu,bWeiweiWang,cJunqiangZhao,aAnjieDongadand1iandongDengaaDepartmentof

2、Po1ymerScienceandTechno1ogyandKey1aboratoryofSystemsBioengineeringoftheMinistryofEducation,Schoo1ofChemica1EngineeringandTechno1ogy,TianjinUniversity,Tianjin300072,ChinabTianjinKey1aboratoryofMo1ecu1arNuc1earMedicine,InstituteofRadiationMedicine,ChineseAcademyofMedica1ScienceandPekingUnionMedica1Co1

3、1ege,Tianjin,ChinacTianjinKey1aboratoryofBiomateria1Research,InstituteofBiomedica1Engineering,ChineseAcademyofMedica1ScienceandPekingUnionMedica1Co11ege,Tianjin300192,ChinadCo11aborativeInnovationCenterofChemica1ScienceandEngineering(Tianjin),Tianjin300072,China摘要：Theabi1itytotunethedegradationrateo

4、fabiodegradab1epo1ymer,whichcanachieveprecisespatiotempora1contro1ofdrugde1ivery,isofconsiderab1einterestforbiomedica1app1ications.Inthisstudy,aseriesofamphiphi1iccopo1ymers,methoxypo1y(ethy1eneg1yco1)-b-po1y(2,4,6-trimethoxybenzy1idene-1,1,1-tris(hydroxymethy1)ethanemethacry1ate)-co-2-(diisopropy1a

5、mino)ethy1methacry1ate)(mPEG-b-P(TTMA-co-DPA),PETD)withdifferentnumbersofDPAunits,weresynthesizedbyReversib1eAdditionFragmentationChainTransfer(RAFT)copo1ymerization,inwhichDPAcontainingtertiaryamineswereintroducedtoadjustthehydro1ysisbehaviorofcyc1icbenzy1ideneaceta1s(CBAs).Themo1ecu1arstructureand

6、thechemica1compositionofPETDwerecharacterizedbyIHNMRandge1permeationchromatography(GPC).PETDcou1dse1f-assemb1eintostab1enanopartic1eswithwe11-definedspherica1morpho1ogyanddisp1ayedhighdrug1oadingcapacitywithdoxorubicin(DOX)asthedrugmode1.Thehydro1ysisbehaviorofCBAsinthePETDNPswasinvestigatedby1JV/vi

7、sspectroscopyunderdifferentpHva1ues.ComparedwithPETD-ObearingnoDPAunit,thehydro1ysisrateofPETD-3withmoreDPAwasfaster,whi1ePETD-1with1essDPAhydro1yzedmuchs1ower,whichindicatedthattheintroductionofDPAhadanamphotericeffectonthehydro1ysisbehaviorofCBAsunderacidicconditions.Inaddition,theinvitrore1easeof

8、DOXwasa1soinvestigatedunderdifferentpHconditionsandtheresu1twasinaccordancewiththehydro1ysisresu1t.Furthermore,theresu1tsoff1uorescencemicroscopydemonstratedimprovedinterna1izationofDOX-IoadedPETD-3NPsinHepG-2ce11swithrapidDOXre1easeintrace11u1ar1y,whichshowedconsiderab1ecytotoxicityagainstHepG-2ce11s.