analysis_method_依普同IPRODIONE异菌脲.docx

《analysis_method_依普同IPRODIONE异菌脲.docx》由会员分享，可在线阅读，更多相关《analysis_method_依普同IPRODIONE异菌脲.docx（2页珍藏版）》请在第一文库网上搜索。

1、ana1ysismethodofIprodione:1.Scope:Thismethodisapp1icab1etoana1yseactiveingredientsofIprodioneWPandSC2.Testmethod:HP1C2.1 insta11ation:2.1.1 HighPerformance1iquidChromatography:2.1.1.1 detector:UVdetector(referredtoasUV).2.1.1.2 chromatographyco1umn:reversephaseco1umn,4.6mm250mm(ID1),BDSHypersi15mC18

2、,orequiva1ent1eve1.2.1.2 u1trasonicdevice(frequency40-50KHz),2.2 Reagents:2.2.1 StandardIprodione12.2.2 interna1standard:Propiophenone,AR.2.2.3 Methano1:HP1Cgradeso1vent.2.2.4 acetonitri1e:HP1Cgradeso1vent.2.2.5 deionizedwater(18.0M-cm,by0.2mmembranefi1ter).2.2.6 acetate(Aceticacid):HP1Cgradeso1vent

3、.2.2.7 Sodiumacetate(Sodiumacetate):AR.2.2.8 pH4,5bufferso1ution:1gsodiumacetatedisso1vedin11deionizedwater,adjustthepHto4.5 (aceticacidabout0.9m1).4.6 .9Di1utionso1vent:pH4.5buffer+acetonitri1e(40+60,v/v).2.3 Theequipmentandmateria1s:2.3.1 vo1umetricf1ask:10m1,50m11100m1.2.3.2 vo1umetricpipette.2.3

4、.3 0.2mny1onmembrane.2.4 PreparationofstandardstockSo1utionsandinterna1standardstockSo1utions:1000gm1Weighabout505mgIprodionestandard(accurateto0.1mg)toa50m1vo1umetricf1askanddi1utewithacetonitri1etothemark.Weighabout505mgPropiophenone(ARstandard)(accurateto0.1mg)toa50m1vo1umetricf1askanddi1utewitha

5、cetonitri1etothemark.2.5 Prepareationofstandardca1ibrationcurvePut0.5,1.0,1.5,2.0,2.5m1standardstockSo1utioninto10m1vo1umetricf1askrespective1y.Add3.0m1interna1standardSo1utions,Di1utewithDi1utionso1venttothemarktomakefivedifferentconcentrationsviz.50,100,150,200and250ppmstandardso1ution.NumberStd1,

6、Std2,Std3,Std4andStd5.0.2mny1onfi1ter.2.6 Preparationofsamp1eAweightof10010mgIprodionewastakenintoa100m1vo1umetricf1ask,di1utedwith90m1acetonitri1e,sonicatedfor5minutes,returntoroomTemperature,acetonitri1emarktoto100m1,mix,andthentake1.5m1ofthisacetonitri1eso1utionto10m1vo1umetricf1ask,Add3.0m1inter

7、na1standardSo1utions,Di1utewithDi1utionso1venttothemark(fina1concentrationabout150gm1),0.2mny1onfi1ter.2.7 Identificationanddeterminationoftest:2.7.1 Instrumentoperatingconditions:2.7.1.1 wave1ength:220nm.2.7.1.2 mobi1ephase:pH4.5bufferso1ution+acetonitri1e+methano1(350+270+380,v/v/v).2.7.1.3 f1owrate:1.0m1/min.2.7.1.4 Injectionvo1ume:201.