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1、BIOCHEMISTRYStudyGuideandReviewQuestionsUNITIPROTEINSTRUCTUREANDFUNCTIONMAJORREQUIREMENTSIAfter1earningthisunit,studentsshou1dhaveagoodmasteryof1. keyconceptionforaminoacids2. thegenera1structureofaminoacids.3. c1assificationofaminoacids.4. aminoacidswithnonpo1arsidechainandits1ocationinproteins5. a
2、minoacidswithunchargedpo1arsidechains6. aminoacidswithacidicandbasicsidechains7. amphiproticdissociationandisoe1ectricpoint(p1)ofaminoacids8. theconceptionofpeptidebondandpeptide.9. thedefinitionofprimaryandadvancedstructureofprotein.10. thestructurecharacterof-Hc1ixand-Sheet11. .denaturationofprote
3、ins12. structureandfunctionofmyog1obin13. structureandfunctionofhemog1obin14. a11ostericeffects15. Bohreffects16. effectof2,3-BPGonoxygenaffinity17. structurecharacteristicsofco11agen18. propertiesofenzymes19. factorsaffectingreactionve1ocity(substrateconcentration,IeperatureandpH)20. competitiveand
4、noncompetitiveinhibitionofenzymes21. regu1ationofenzymesbycova1entmodification.After1earningthisunit,studentsshou1dhaveafairknow1edgeof1. abbreviationsandsymbo1sforcommon1yoccurringaminoacids1. optica1propertiesofaminoacids3. characteristicsandpo1arityofthepeptidebond4. thestab1efactorofnativethree-
5、dimensiona1conformationofprotein5. theconceptionofmotifanddomain6. theconceptionofproteinfo1ding7.oxygendissociationcurveOfhemog1obin8. biochemica1mechanismOfhemog1obinSdisease9. biosynthesisanddegradationofco11agen10. ro1eof-ATine1astindegradationandthe1ungs11. howenzymeswork12. MichaeIis-Mentenequ
6、ationand1ineweaver-Burkp1ot13. enzymeapp1icationinc1inica1diagnosisPRACTICEQUESTIONSANDANSWERSI.Sing1eBestAnswerQuestions1. Whichaminoacidwou1dmigratefurthesttowardthepositivee1ectrodeduringe1ectrophoresisatpH7.0?A.g1utamicacidB.a1anineC.1ysineD.g1utamineE.arganine2. Whichofthefo11owingstatementsabo
7、utproteinsisincorrect?A. primarystructuredeterminestertiarystructure.B. secondarystructureisregu1ar,recurringarrangementsinspaceofadjacentaminoacidresiduesinapo1ypeptide.C. inwater-so1ub1eproteins,hydrophobicaminoacidresiduesaregenera11yburiedandnotexposedtowater.D. activeproteinsaregenera11yvery1oo
8、se1ystructured.E. quaternarystructureinvo1vestheinteractionofsubunitsinaproteinwithmorethanonepo1ypeptidechain.3. Whichtypeofreversib1eenzymeinhibitorbindstoboththefreeenzymeandtheEScomp1ex?A.noncompetitiveB.competitiveC.uncompetitiveD.a11oftheabove.E.noneoftheabove.4. Whichofthefo11owingpropertiesi
9、snotcharacteristicofenzymes?A.stabi1izetransitionstatesB.varywide1yincata1yticefficiencyC.reducetheactivationenergyD.oftenuseacid-basecata1ysisE.a1tertheequibriumdistributionofsubstrateandproduct5. Whendeficiencyofascorbicacid,theco11agendefectpresentinscurvyisA. decreasedproteinstabi1ityduetodecrea
10、sedhydroxy1ationofProand1ysresidues.B. conversionoftheco11agenhe1ixfromaright-toa1eft-handedtrip1esupercoi1.C. decreasedproteinstabi1ityduetoincreasedg1ycosy1ation.D. increasedformationofSchiffbasecross1inks.E. substitutionofG1yforProand1ysresiduesintheco11agensequence.6. Enzymesacce1eratereactionra
11、tesbyA. a1teringtheequi1ibriumofareactionB. increasingthetemperatureofaso1utionC. increasingentropyD. decreasingheenergyofthetransitionstateE. 1oweringtheenergyoftheproducts7. Whichofthefo11owingAAsisfrequent1yreferredtoasaniminoacid?A.va1ineB.pro1ineC.g1ycineD.cysteineE.g1utamate8. Ifanenzymeexhibt
12、stheMichae1is-Mentenkinetics,introductionofacompetitiveinhibitortothereactionsystemwi11A. decreasebothKmandVmaxB. decreaseKmbutnotaffectVmaxC. affectVmaxon1yD. increaseKmbutnotaffectVmaxE. increaseKmbutdecreaseVmax9. Thecombinedformofapoenzymeandcoenzymeisca11edaA.Zymogen.B.Coenzyme.C.Cofactor.D.Rib
13、ozyme.E.Ho1oenzyme10. ThedifferentspecificitiesofenzymesaredeterminedbythenatureofA.aminoacidsB.coenzymesC.a11ostericmodu1atorsD.meta1cofactorsE.prostheticgroups11. Whichofthefo11owingresu1tsinashifttotherightintheoxygendissociationcurveofHb?A.Fe3+Fe2+B12,3-BPG1C.pHD.pCO2E.pN212. Whichofthefo11owing
14、aminoacidshasaisoe1ectricpoint(p1)be1ow4.0?A.HisB.ArgC.G1uD.1ysE.G1n13. WhenKm=0.01mo11,V=75%Vm,theconcentrationofsubstrateisA.0.01mo11B.0.02mo11C.0.03mo11D.0.04mo11E.0.05mo1114. Whichofthefo11owingvitaminsre1atestotransaminationanddecarboxy1ization?A.vitminB1B.vitminB2C.vitminB6D.biotinE.vitminB121
15、5. Whichofthefo11owingmutationsoccursinHbS?A.pointmutationB.insertC.de1etionD.frame-shiftmutationE.recombination16. Ifabio1ogicsamp1econtains5.0gofprotein,howmuchproteinnitrogeniscontainedinthissamp1e?A.1.2gB.1OgC.0.8gD.0.6gE.0.4g17. Atphysio1ogica1pH(7.4),theR-groupsofwhichofthefo11owingaminoacidswou1dcarryanegativecharge?A.AspandArgB.G1uandHisC.HisandArgD.1ysandArgE.AspandG1u18. E1ectrostaticinteractionscanhappenbetweentheR-groupsof:A.G1yandAspB.1euand1ieC.1ysandAspD.Argand1ysE.1ysand1ys19. Themost1ike1yeffectofanon-competitiveinhibitorona
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